Second of two parts. Read Part 1.

EAST LAKE — Before guardrails were installed about 20 years ago along U.S. Highways 64 and 264 in rural northeastern North Carolina, residents avoided driving at night in fear of striking a large animal and then sliding unseen into the abyss of a roadside canal.

Even now, with the barriers in place, locals know to drive with caution through the dark wilds of Alligator River National Wildlife Refuge, scanning the edge of the forest for glowing eyes or sudden movements of creatures on night hunts — raccoon, possum, bobcat, fox, bear, deer, coyotes and red wolves.

Vehicle strikes are a serious hazard to humans and animals, but they can be especially devastating to the recovery of the endangered wolves that number only about 22 in the wild, 18 of which are collared and within the 1.7-million-acre management area encompassing public and private land in Beaufort, Dare, Hyde, Tyrrell and Washington counties.

When a wild red wolf is killed, the loss can destroy the cohesion of a pack, creating a negative impact on reproduction that is so critical to the species’ survival.

Last year, for example, in two separate instances, wolves from the same pack were struck and killed on U.S. 64, said wildlife biologist Joe Madison, manager for the U.S. Fish and Wildlife Service’s Red Wolf Recovery Program.

Madison told Coastal Review that one of the males and one of the pups were killed. “So that family group kind of got messed up, and we ended up capturing and placing the female for that family group back in captivity.”

With the loss of her mate, Madison explained, the female had started wandering to a different area and creating issues, such as getting into chickens that made her no longer suitable for the wild. “But it was going well until that mortality of the male and one of the pups, and then it kind of went downhill from there.”

After years of study in the early 2000s, the North Carolina Department of Transportation had developed plans to construct numerous wildlife crossings along U.S. 64 in Dare and Tyrrell counties as part of a proposed 27.3-mile-long road widening and bridge-replacement project. The department has since dropped the widening project, but $110 million provided recently by the Bipartisan Infrastructure Law allowed NCDOT to replace the 60-year-old Lindsey C. Warren Bridge over Alligator River. That $270 million project, which began this spring, will include wildlife crossings and under-road tie-ins at both ends of the bridge.

But it’s not enough, conservation groups say. Granted, more wildlife crossings would be costly to build in Alligator River’s swampy land, but considering the enormous investment that’s been put into the life of each red wolf in the interest of restoration of a unique species, these groups contend they’re worth it.

“That’s why one of the things we’re stressing this year is trying to make progress on getting (assistance from) NCDOT, who’s making great strides in the wildlife road crossings department,” Ron Sutherland, chief scientist at the nonprofit Wildlands Network, told Coastal Review recently. “We want them to try to put in for federal grants to build wildlife crossings and fencing on 64 through the refuge in particular.”

Sutherland had connected with an anonymous donor who recently pledged $2 million in matching funds for a grant to fund wildlife crossings in the refuge to protect red wolves, and the nonprofit Center for Biological Diversity stepped in to help raise the match, he said in recent email.

“I’m working directly with NCDOT to try to bring a big proposal for U.S. 64 to Federal Highways, which can only happen if we have enough nonfederal matching funds to work with, he said, adding that the state would have to provide a 20% match to the Federal Highway Administration money.

Although the costs versus benefit of keeping red wolves away from vehicle tires is clear, he said, wildlife crossings through a refuge teeming with wildlife would provide plenty of benefits to every creature dashing, hopping, galumphing, scurrying, slithering or crawling across the highway.

“That stretch of Highway 64 through the refuge and through the Alligator River game lands, it’s got to be up there in terms of national priorities for reducing roadkill in terms of the sheer numbers of wildlife,” Sutherland said. “There were like tens of thousands of dead animals that they recorded in the DOT-funded study. And so it’s definitely not just the wolves, but bears and deer and bobcats and so many turtles, so many snakes … that I’ve seen dead on that road. Nobody wants to see that.”

According to the draft environmental impact statement for the then-proposed widening project, 36% of all crashes and 77% of night crashes on the two-lane road were because of animals. Five crashes occurred within a milelong stretch in Tyrrell County about a mile west of the bridge.

Between July 1996 to June 1999, the fatal crash rate for the project area was 4.13 crashes per 100 motor vehicle miles. After the guardrails were installed along the canals on U.S. 64, the fatal crash rate went down to 1.02 per 100 miles.

The proposed widening had called for about 11 overpasses or underpasses and dozens of smaller structures for amphibians, reptiles and small mammals. Four wildlife crossings that were installed decades ago off U.S. 64 between Columbia and Plymouth had been shown to be about 90% effective, a U.S. Fish and Wildlife Service biologist had said in 2013. Designed with 6- to 8-foot-high fences at the road edge and both sides of the opening, the fence corrals animals toward underpasses, culverts or a bridge.

Travis Wilson, a biologist with the North Carolina Wildlife Resources Commission Habitat Conservation Division, said that the east and west sides of the proposed 3.2-mile Alligator River bridge replacement will be lengthened to accommodate culverts for fencing and wildlife passage.

Based on his years of monitoring the commission’s wildlife crossings, Wilson said he expects that all species will use the passages, although white-tailed deer tend to be more skittish.

“I have documented most every large mammal, medium-sized mammal, in North Carolina using culverts fairly frequently, from black bear to coyotes, on down,” he told Coastal Review.

If funding is found for additional crossings beyond the bridge, he said they would be designed in different sizes for different species. Vegetation at the crossings, and the fencing would need to be maintained, and some areas in front of passages would need a timber pole “bridge” over a canal.

“That’s really what the crossings are there for,” he said.  “It’s not for a single species — it’s to reduce wildlife mortality by vehicles … to make the highway more permeable to all wildlife.”

While the recovery team would welcome wildlife crossings, the staff’s focus will remain on keeping wild-born and captive-bred wolves who have been introduced into the wild away from any human interactions and activity whatsoever. The less habituated wolves are to humans, the better for both species. The staff also takes pains to minimize contact as much as possible, Madison said, and when handling is necessary, it is done as gently as possible, with voices low and no petting.

In addition to using a hand-held antenna to keep track of the collared wolves, which wear lightweight GPS devices on reflective collars, or for some, smaller VHF radio devices, there are more than 55 remote sensing cameras to see who is where and when.

GPS collars, which cost about $2,000 and weigh 1.3 pounds, cannot exceed 4% of the animal’s body weight. The VHF collars are lighter but don’t send points from satellites.

A red wolf known as No. 2191 was recently sighted in the Milltail area of the Alligator River refuge. Madison said that the young male’s fear of people gives him a better chance to avoid becoming one of the unfortunate number of casualties suffered by red wolves from too-close encounters with people.

Madison held a small radio telemetry antenna during a visit to the Milltail area in late April. A steady beep revealed that the wolf — or more specifically his GPS collar — was close but too far away to see without field glasses. The wolf was born at Wolf Haven International in Washington state, one of the 50 zoological institutions and wildlife centers that participate in the captive-breeding program that is critical to repopulating the species in the wild.

When 2191 — the animals purposely are not named — was deemed ready for life in the wild, he was transferred to Alligator River.

“They did an excellent job,” Madison said, referring to Wolf Haven, “because he wants nothing to do with people.”

After his arrival, 2191 was placed in an acclimation pen before being released on Jan. 29 to meet a female who had come into heat, “in the hopes that they could become a pair,” said Madison.

The good news is that it appears that the handsome wolf is the father of a litter of eight pups born in the refuge in May. It’s the third year in a row that the Milltail pack has produced a litter, and this was the first sired by 2191. The previous breeding male that had sired two litters was killed by a vehicle last year.

Madison said he understands why zoos and conservation centers name the wolves, but it’s against the recovery team policy. The studbook number that is assigned to each animal identifies them in sequence that is vital management information.

American red wolves once had an enormous range in the Southeast and along the Gulf Coast. But because of habitat loss and hunting, the population collapsed. The red wolf was listed as endangered in 1973 and declared extinct in the wild in 1980. In 1987, four pairs of captive-bred wolves were released at Alligator River refuge.

Innovative management practices, such as pup fostering and coyote sterilization programs, grew the population, and by 2010, there were about 130 red wolves in the wild. But politics and funding shortages led to management cuts, and the population plummeted to seven before a federal judge ordered the program to resume in 2021.

Starting over has had its challenges. When 11 captive-born wolves were released in 2022, three wound up dead from gunshots and five were killed by vehicles. In the last year alone, four wolves have been killed by vehicles.

Still, the new litters provide hope, and the restored pup fostering practice — where a captive-born pup is slipped into a wolf den with a litter of pups about the same age — has been successful. So has the renewed coyote sterilization program, which allows hormonally-intact coyotes to hold territory, keep out fertile coyotes and prevent hybrids.

From November until March, the recovery team is kept busy doing captures to collar older pups, perform health check on the mature wolves and sterilize coyotes. There are 16 pens in the Sandy Ridge area, each double-fenced, but only 13 are currently usable. Interns and other staff enter the pen to water and feed the wolves and check on them. At that point, the wolves either go to the farthest distance and pace, or they go to their den box. The never try to escape.

“They don’t want to come near you,” Madison said. “They’re very nonaggressive.”

The pens are especially useful in letting wild wolves visit the captive wolves and start making friends. Recovery staff can watch with the remote-sensing cameras for signs that courtship may be blooming. Once they’re let free, all bets are off.

“We’ve had bonded pairs that came from captivity,” Madison recalled. “They were bonded in captivity, had had previous litters together, they had a litter in the pen, and they still left each other when we opened it up. It was like, ‘Now that I have options, you ain’t it!’”

Sutherland said that he is encouraged that the red wolf population is rebounding and that wildlife crossings are a critical component in its recovery. Healthy numbers of red wolf packs also would go far in pushing out a lot of the opportunistic coyotes and raccoons that swooped into vacated wolf territories, he said.

As they’ve done out west, he said, wolves can keep other species in check not just by eating them, but also by creating a climate of fear that works for the good of the entire ecosystem.

“So that’s the value of having the wolves back,” he said. “Not only are they the only thing that seems to control coyotes, but they also do kill the raccoons and we think that’s important from the standpoint of bird populations.”

“The red wolf was a success story of the Endangered Species Act, and it’s been saved from extinction,” Sutherland said. Now the question is whether the program can rebuild, without the apex predator being plowed down on a strip of asphalt.

Note: Coastal Review will not publish Wednesday in observance of Juneteenth National Independence Day, a federal holiday.

First of two parts.

EAST LAKE — From a half-mile away, the red wolf was a blur on the flat farmland within Alligator River National Wildlife Refuge. Through binoculars, the young male was strikingly muscular, striding with confidence on the dirt access road, seemingly unperturbed by the spying humans.

“He’s a big guy — yeah, he’s close to 80 pounds,” said Joe Madison, manager for the U.S. Fish and Wildlife Service’s Red Wolf Recovery Program, while peering through his field glasses in late April from the cab of his truck. “If we got closer, he’d definitely start running, but I don’t want to do that.”

Madison, a wildlife biologist who has had earlier stints with grizzly bear and gray wolf management, is keenly focused on conservation and protection of the only wild red wolves in the world. He knows that the wolves’ instinctual fear of people is critical to their survival. The two biggest contributors to wild red wolf mortalities are directly related to interactions with humans: The first is intentional killing by gunshot or poisoning, the second is vehicle strikes.

After establishing cooperative programs with landowners and others in the community to prevent wolf shootings, officials with the refuge are now working with the North Carolina Wildlife Resources Commission and the North Carolina Department of Transportation on constructing wildlife crossings at both ends of the planned replacement bridge over the Alligator River on U.S. Highway 64 between Tyrrell and Dare counties.

The hope is that, beyond the bridge project, funding also will be available to build numerous crossings along U.S. 64, said Travis Wilson, a biologist with the North Carolina Wildlife Resources Commission Habitat Conservation Division.

“It’s kind of a standalone discussion on wildlife improvements in highway permeability improvements,” Wilson told Coastal Review. “It’s outside the scope of a highway project.”

The Center for Biological Diversity announced last week that an anonymous donor had pledged a $2 million match of other donations toward wildlife crossings across U.S. 64, which bisects the Alligator River and Pocosin Lakes wildlife refuges. If the additional $2 million can be raised by the nonprofit center and its supporters by the target date of Aug. 1, it could leverage an additional $16 million in federal funds.

Funding for $350 million in grants was provided in the Wildlife Crossings Program, established in the 2021 Infrastructure Investment and Jobs Act.

A million wildlife-vehicle collisions occur in the U.S. annually, costing more than $8 billion and resulting in thousands of injuries and hundreds of fatalities, according to the Federal Highway Administration website.

A second round of Highway Administration discretionary grants will be opened this summer under the Wildlife Crossing Pilot Program with the stated mission of reducing wildlife-vehicle collisions while improving habitat connectivity for terrestrial and aquatic species.

Marissa Cox, the Western Regional Team lead with NCDOT’s Environmental Policy Unit, said that her team intends to apply for a grant, but it does not know yet what the total cost of the project would be. NCDOT is using information from the proposed — and since dropped — U.S. 64 widening project design plans to try to estimate costs for the structures, she said in an interview.

“It’s very competitive, and there’s not a lot of funding,” Cox said about the grant program.

During the first round, she recalled, the amount of project applications far exceeded the available funds.

Although Cox said there are about 26 wildlife crossings in North Carolina, Wilson said that when standalone structures are included, there are “dozens and dozens” of crossings.

As part of a wildlife stewardship memorandum of understanding signed in March 2023 with Wildlife Resources, NCDOT is currently compiling information and Global Positioning System data on all the crossings that it has committed to, designed and constructed, she said. The agencies are also finalizing a joint Wildlife Crossing Guidance document to be made available online.

With U.S. 64 and other less-traveled highways cutting through the 1.7 million-acre management area encompassing public and private land in Beaufort, Dare, Hyde, Tyrrell and Washington counties, the wildlife crossings could benefit not only the survival of the red wolves, but also the taxpayer who is supporting the recovery program.

A recently updated red wolf management plan estimated costs of $328 million over 50 years, and that does not include the millions spent over the decades since the wolf conservation program began.

The red wolf had once roamed much of the Southeast, but overhunting and habitat loss decimated its population. In 1973, the species was listed as endangered under the Endangered Species Act. Fish and Wildlife, the agency charged with implementing the Endangered Species Act, first listed the red wolf as endangered in 1967, and it was declared extinct in the wild in 1980.

As part of an effort in 1987 to restore the species in the wild, four pairs of captive-bred red wolf pups, offspring of the few remaining from the wild population captured earlier in Louisiana, were released at Alligator River National Wildlife Refuge.

After a series of setbacks beginning around 2010 — with the wild population plummeting from as much as 130 to seven — the recovery program has been renewed and reinvigorated since 2022.

Currently, there are 18 known and collared red wolves and a total of about 20 to 22 wolves in the wild and 263 in the captive-breeding population.

Wildlife crossings have been studied, planned and – sometimes – built along roadways in northeastern North Carolina, but in coming years they are to be a more significant part of the focus on conservation of the fragile population.

“Wildlife crossings along one of North Carolina’s most dangerous highways are crucial to protecting the world’s most endangered wolf,” stated Will Harlan, southeast director the Center for Biological Diversity.

In a study done for NCDOT by the Virginia Polytechnic Institute and State University and completed in 2011, hair samples caught on a barbed fence were from 890 black bear crossings from March 2009 to March 2011 in the 147,432-acre Alligator River Refuge. The Virginia Tech study also found that 15 GPS-collared bears crossed the highway 99 times. In addition, 170 white-tailed deer, 200 bobcats and raccoons, and an additional 260 bear were caught on camera.

Surveys of roadkill from November 2008 to July 2011 showed eight deer killed. Between January 1993 and July 2011, factoring in historical data, there were 63 bear, 75 bats, 82 small mammals, 134 mid-sized mammals, 1,153 birds, 4,014 reptiles and 7,498 amphibians killed on the road. And in 2012, refuge biologists reported that 11 bear were hit by vehicles, not including those who ran off into the woods after being struck.

Data from the Virginia Tech study will be used to guide project estimates for crossings through the refuge, Cox said.

The Fish and Wildlife Service, in its February red wolf recovery program five-year status review, said that between 1987 and 2013, vehicle-wildlife collisions resulted in 34% of all mortalities related to humans; and there were 11 vehicle-related mortalities between 2019 and 2023.

“This mortality level would be expected to increase as habitat becomes more fragmented by roads and with increasing human traffic that would be expected with increased development,” the report said. “Additionally, this threat would also likely increase with increases in the population size of red wolf.”

Madison said that there is now orange reflective material on the GPS collars placed on the wild wolves to increase their visibility at night. There are also roadside mobile electronic message signs to warn drivers on all the highways.

Any wildlife crossings that are proposed separately from an NCDOT project, which would absorb some of the costs, will “not be inexpensive,” Wilson said.

“We have been successful in putting underpasses in coastal North Carolina in various places,” he said. Swampy Alligator River, with its numerous roadside canals, “has its own unique features and soil conditions,” Wilson said.

“That’s a big part of the conversation, building in the soil types that are out there, the fill and the engineering that have to go in place there. And as you know, anything that becomes more complex, the dollar figures begin to increase with that complexity,” he said.

“When it comes to the mammals that we’re talking about, if your structure is designed correctly and located correctly, and you have appropriate fencing, then you’re going to have mammals find those crossings and use those crossings,” he said. “And once they start, they’ll keep using them.”

The crossings provide habitat connectivity, as Wilson explained it.

After the New York Times recently published an article about wildlife crossings that included video of wildlife using an overpass, public interest in wildlife passages nationally increased dramatically.

“My phone blew up with reporters and the public wondering when is North Carolina going to do these things. And it felt like I spent a year on the phone every other day explaining to people that North Carolina has been doing it for two decades,” Wilson said. “The documents are memorializing a lot of what we’ve done but also will be good tools to give to people who have interest.”

Next in the series: Wildlife crossings dovetail with red wolf conservation science.